1.Y. Torrejon(1),*, A. Troco(1), C.A. Sousa(2), F. Fortes(2), J. Pinto(1), P. Salgueiro(1)
(1) Global Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, Lisboa, Portugal;
(2) Programa Nacional de Controlo da Malária, Ministério da Saúde, Luanda, Angola
Human malaria is a parasitic disease caused by five species of the genus Plasmodium that are specifically transmitted by Anopheles mosquitoes. The importance of the gene TEP1 in the mosquito immune response to Plasmodium infection has been demonstrated [1,2]. This gene has four alleles, two of them associated to the resistance against Plasmodium infection (R1 and R2) and two associated with susceptibility to infection (S1 and S2).
The present work aimed at genotyping the TEP1 gene in the malaria vector Anopheles coluzzii collected in Luanda, Angola, in 2006 and 2010. For the purpose, a PCR-RFLP assay was performed using three different restriction enzymes (Hind III, BamH I and BseN I) in DNA samples from mosquito specimens previously identified to species by PCR.
Nineteen specimens from 2006 were all genotyped as S1/S1 (susceptible). Forty-five samples from 2010 were analyzed and the genotype frequencies were 80.1% S1/S1, 4.4% S1/S2, 13.3% S1/R2 and 2.2% S1/R1,
These preliminary results show the presence of R1 and R2 alleles only in the sample of 2010, suggesting a recent introduction/selection of Plasmodium-resistance alleles in this vector population. However, more samples need to be analyzed to confirm these results.
 Blandin et al. (2004). Cell 116: 661-670.
 White et al. (2010). PNAS 108: 244–249