A.Fox Ramos(1),*, C. Pavesi(1), J.-F. Gallard(2), V. Dumontet(1), M. Litaudon(1), P. Champy(1), M. A. Beniddir(1)
(1) Équipe “Pharmacognosie-Chimie des substances naturelles” BioCIS, Univ. Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.-B. Clément, 92290 Châtenay-Malabry, France
(2) Centre de Recherche de Gif, ICSN, CNRS, LabEx LERMIT, 1, avenue de la Terrasse, 91198 Gif-sur-Yvette, France
*firstname.lastname@example.org, Tel: +33 778953238
Throughout the six past decades, Apocynaceae plants have drawn the interest of generations of chemists due to their content in Monoterpene Indole Alkaloids (MIAs), a therapeutically valuable class of natural products. [1, 2] Recent advances in bioinformatics and analytical chemistry, particularly in mass spectrometry, have enhanced the field of natural product discovery. Recently, molecular networking is emerging as a promising computer-based approach to visualize and organize tandem MS/MS data sets and to automate database searches for secondary metabolite identification within complex mixtures. In that context, this dereplication technique has been exploited by our team to undertake the phytochemical study of three New-Caledonian Alstonia spp.  Using this approach, we have sought to develop an efficient tool able to prioritize identification and isolation of new MIA-type alkaloids from complex plant natural extracts, with the objective to create a large chemical library for biological screening. This tool is based on the implementation of an in-house MIAs MS/MS DataBase (MIADB), which benefited from our historical collection of natural alkaloids. 
The present communication describes the dereplication of eighteen known alkaloids from the dried stem barks and leaves of Alstonia balansae Guillaumin, Alstonia vieillardii Van Heurck & Müll.Arg., and Alstonia quaternata Van Heurck & Müll.Arg., as well as the MS/MS guided isolation of six new alkaloids.
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 A. E. Fox Ramos, C. Alcover, L. Evanno, A. Maciuk, M. Litaudon, C. Duplais, G. Bernadat, J.-F. Gallard, J.-C. Jullian, E. Mouray, P. Grellier, P. M. Loiseau, S. Pomel, E. Poupon, P. Champy, M. A. Beniddir, J. Nat. Prod., 80, 1007-1014, 2017.